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dc.contributor.authorGonzález Feliciano, José A.
dc.contributor.authorGonzález Vargas, Carlos I. (Consejero)
dc.date.accessioned2015-11-21T21:25:46Z
dc.date.available2015-11-21T21:25:46Z
dc.identifier.urihttp://hdl.handle.net/123456789/2315
dc.description.abstractAn important aspect of the regulation of gene expression in eukaryotes is the post-transcriptional control. Adenosine/Uridine-rich elements (AU-rich elements or AREs) within the 3'-untranslated region (3'-UTR) of mRNAs play a crucial role in the post-transcriptional regulation by affecting mRNA stability and/or translation. Cells involved in the immune response produce several pleiotropic lymphokines that contain AU-rich elements in their mRNAs. Interlukin-3 is one of these lymphokines that harbor an AU-rich element in its 3'-UTR. The aberrant expression of IL-3 is related to cancer, sickle cell disease and asthma, among others. At present, little is known about the ARE-mediated post-transcriptional regulation of human IL-3. In order to fill this scientific gap, the main focus of this dissertation is to understand the human IL-3 ARE-mediated post-transcriptional regulatory pathway. This manuscript is divided in four chapters. Chapter I consists of an introduction to the ARE-mediated post-transcriptional regulatory mechanism. Chapter II focuses on the characterization of the IL-3 ARE post-transcriptional role. Chapter III describes and characterizes the ARE-BPs that recognize the hIL-3 ARE and their roles in mRNA stability and translation. Finally, Chapter IV elucidates the role of T-cell activation in the hIL-3 ARE-mediated post-transcriptional regulation and its effects in the IL-3 ARE-binding protein complexes. Altogether, the results presented in this dissertation provide new evidence regarding the IL-3 ARE-mediated post-transcriptional regulation in human cells.
dc.language.isoen
dc.subjectPost-transcriptional Regulation
dc.subjectmRNA Decay
dc.subjectRNA regulatory elements
dc.subjectRNA binding proteins
dc.subjectTranslational Control
dc.subjectCis-acting Elements
dc.subjectAu-Rich elements
dc.titleIdentification and Characterization of Au-Rich Elements and Trans-Acting Factors Involved in the Posttranscriptional Regulation of Human Interleukin-3
dc.typePhD Dissertation


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